Prof. (Dr.) Kapil Lawrence

PROFESSIONAL SUMMARY EXPERTISE INTELLECTUAL PROPERTY PROJECTS PUBLICATIONS CONFERENCES TEACHING EXPERIENCE OTHER

Professor

+91-+919839123574

kapil.lawrence@shuats.edu.in

Additional Responsibilities

Head, Department of Molecular & Cellular Engineering

Contact Details

+91-+919839123574

kapil.lawrence@shuats.edu.in

Department of Biochemistry & Biochemical Engineering

Educational Details

Doctorate

Ph.D

Lucknow University,
Lucknow

Graduated in 1996

MPHIL

M.Phil

Lucknow University,
Lucknow

Graduated in 1989

PG

NET

UGC

Qualified in 1989

M.Sc

Lucknow University,
Lucknow

Graduated in 1986

UG

B.Sc

Lucknow University,
Lucknow

Graduated in 1984

Professional Summary

Prof. (Dr. ) Kapil Lawrence did his M.Sc., (1986) M.Phil. (1989) and Ph.D. (1996) from Lucknow University in Biochemistry. He has also qualified National Entrance Test (NET) (CSIR-UGC) in 1989 for award of JRF. After a brief teaching stint as part-time lecturer at Isabella Thoburn College, Lucknow from September 1995 till March 1996. He joined Sam Higginbottom Institute of Agriculture, Technology & Sciences Deemed University then known as Allahabad Agricultural Institute, Allahabad in 1996 as Assistant Professor. Currently, he is Professor & Head, Dept. of Molecular & Cellular Engineering. He also served as Head, Dept. of Biochemistry & Biochemical Engineering from July 2009 till July 2012. From 2006-2008 he was on deputation as Associate Professor at Dental College, Zleiten, Al Mergeb University, Al Khums, Libya teaching Biochemistry to first year students of Dental College. Research details, list of publications etc. is given under employee profile.

Area of Specialization

Biochemistry

Expertise

(i) 1986-87- Junior Research Fellow (JRF) – Division of Membrane Biology, Central Drug Research Institute, Lucknow, UP., INDIA. The work involved:

(a) Preparation, Purification and identification of proteins from ghosts in malarial parasite infected and normal RBC. Identification of membrane proteins using SDS-PAGE.

(b) Preparation of Phosphatidylcholine and lyso-phosphatidylcholine from egg yolk through several purification procedure including chromatography and chemical modification.

(c) Preparation of pure uni-lamellar and multi-lamellar liposomes by ultracentrifugation and gel-filtration chromatography as well as lyophilization and use labeled with probes to be used as in vitro drug vehicles.

(ii) 1988-95: Department of Biochemistry, Lucknow University, Lucknow UP., INDIA as JRF and Senior Research Fellow NET-CSIR for M.Phil and Ph.D under Prof. (Dr.). P.C. Misra. The work involved:

(a) Hydroponic culture of Cicer arientum seedlings under the conditions of mineral deficiency and sufficiency in a sterile environment.

(b) Measurement of Respiratory rate and pH fluxes with highly sensitive instrument. Measurement of trans-plasma membrane electron transport activity using artificial electron acceptors such as ferricyanide, hexachloroiridate and ferric-EDTA.

(c) Isolation of purified plasma membrane vesicles (pmv) from root tissue by aqueous polymer two-phase separation technique.

(d) Purity studies encompassed spectrophotometric assay of various marker enzymes in the crude (microsomal fraction) and purified preparation pmv viz. K+ stimulated Mg2+ ATPase, NO3- sensitive ATPase azide sensitive ATPase, cytochrome c oxidase, NAD (P) H-cytochrome c reductase. Biological detergents (s) were used to determine sideness of pmv.

(e) Morphology of the fractions was studied with transmission electron microscopy.

(f) Trans-plasma membrane redox activity measurement was determined by assaying the following enzymes (spectrophotometrically): NADH: glutathione oxidoreductase.

(g) Determination of Kinetic constants Km and Ki from ATPase Km for NADH:ferricyanide oxidoreductase.

Projects

Prof. ( Dr.) P W Ramteke (PI), Prof. (Dr.) Kapil Lawrence	Characterization of cold-adapted extra cellular enzymes \from micro organisms and their biotechnological applications. 3 years	2003-2006	22,29,000 INR	DBT

Publications

Lawrence, K., Bhalla, P. and Misra, P. C.: Changes in NAD (P) H-dependent redox activities in plasmalemma enriched vesicles isolated from boron and zinc deficient chickpea roots. J. Plant Physiol. Vol. 146 pp. 652-657 (1995). Impact Factor: 2.557
Lawrence K., Bhalla, P. and Misra, P.C.: NADH-dependent redox activities on the external face of plasma membrane vesicles of chickpea roots. J. Plant Physiol. 146, 763-765. (1995) Impact Factor: 2.557
Bhalla, P., Lawrence K. and Misra, P.C.: Plasma membrane bound ferrocyanide oxidase activity in roots of chickpea (Cicer arietinum) seedlings. Ind. J. Mol. Biol. 35, 1229-1232 (1996). ISSN: 0975-1009 (Online); 0019-5189 (Print) Impact Factor: 0.835
Prakash V., Alka and Lawrence K.: Anti-oxidative response to copper induced stress in Cicer arietinum L. Ind. J. Agril. Biochem. 2, 23-26 (2007). ISSN: 0970-6399.
Lawrence, R., Lawrence, K., M. Prasad and Ritika Singh: Antioxidant activity of methanol extract of Zingiber officinale grown in North Indian plains’. The Scientific Temper, 1, 117-120 (2010).
Lawrence, R. and Lawrence, K. (2010);’A new glycoside from the buds of clove grown in North India’ The Scientific Temper, 1, 155-156 (2010).
Gupta G., Tewari S.K., Lawrence, K. and Lawrence, R. (2011): Effect on growth and biodiesel production in fresh water diatoms. Plant Archives, 11, 673-676 (2011). ISSN:0972-5210
Lawrence, R. and Lawrence, K.: Antioxidant activity of garlic essential oil (Allium sativum) grown in North Indian plains. Asian Pacific J. of Tropical Biomed. l, S888-S891(2011). ISSN: 2221-1691
Lawrence, K., Lawrence, R., Parihar, D., Srivastava, R. and Sharan, A.: Antioxidant Activity of Palma rosa essential oil (Cymbopogon martini). Asian Pacific J. of Tropical Biomed. 1, 1- 4. (2012) ISSN: 1995-7645
Kumar, R., Kumar, S. S, Ali, M., Kumar, A, Nath, A. Lawrence, K., Pandit, S. and Singh, J.K.: Bioremedial impact of Withania somnifera on histology and biochemical parameters of kidney of stressed mice. Indo-Global Res. J. Pharma. Sci. 2, 349-352 (2012). ISSN: 2249-1023.
Kumar, R., Kumar, S. S, Ali, M., Kumar, A. Nath, A. Lawrence, K. and Singh, J.K.: Impact of stress on histology and biochemical parameters of liver and kidney of mice. Innov. J. Medical and Health Sci. 2, 63 – 66 (2012). ISSN: 2277-4939
Lal, V. F., Thakur, S., Srivastava, S. and Lawrence, K.: Bioassay evaluation of acute toxicity levels of zinc chloride to an air breathing teleost Channa punctatus (Bloch) (2013): J. Exp. Zool. Ind. 16, 303-306(2013).( ISSN : 0972 - 0030 )
Sharan A., Lawrence K., Lawrence, R.S. and Charan A. A.: Production optimization and partial purification of glucoamylase from A. niger using waste banana peel and wheat bran. The J. of Bioproc.Tech. Photon 97, 157-164(2013). (ISJN: 6383-4392) Impact Index: 5.94.
Alhmadi, N., Lawrence, R.S., Alhmadi, I. and Lawrence, K.: Composition and determination of antioxidant activity of essential oil of Citrus reticulata grown in North Indian Plains. The J. Free Rad. & Antioxid. Photon, 139, 153-159(2013). (ISJN: 9102-2347, Impact Index: 5.13
Singh S., Pandey, A., Sharma, B., Lawrence, K. and Pandit, S.: Imidacloprid induced osmotic fragility in erythrocytes of rats: protective role of Vit.C and tea. IOSR-JESTFT 5, 103-105(2013). (e-ISSN 2319-2402 p-ISSN 23192399)
Pandit, S., Lawrence, K., Singh, A., Singh, S. and Lawrence, R.S.: Cellulase production by Aspergillus flavus and saccharification of wheat straw. Int. J. Sci.& Engin. 4, 1965-1971(2013). (ISSN:229-5518)
Sharma B., Kumar S. S., Siddiqui S., Lawrence, R.S., Lawrence, K. and Chaudhary, M.: Aqueous extract of Azadirachta indica protects the liver from hyperglycemia induced toxicity during diabetes in Swiss albino mice. Intl. J. Pharma. Photon 104, 224-231(2013). (ISJN: 8237-7516, Impact Index: 5.50)
Pandey, A., Gupta, R. K., Lawrence, R.S., Lawrence, K. and Srivastava, R.: Synergistic study of antioxidant potential of different spices and their bioactive constituents. Intl. J of Pharn. Sc. & Res. 5, 1000-05 (2014). (ISSN Online: 0975-8232, ISSN Print: 2320-5148)
Pandit S., Lawrence, K., Singh A. and Bais, S.S.: Saccharification of bagasse, paper waste by cellulase produced from Aspergillus flavus. Int. J. Pure App. Biosci. 2, 16-22 (2014). (ISSN: 2320 – 7051)
Dar, J., Lawrence, R.S., Sikander, D. and Lawrence, K.: Antioxidant activity of essential oil extracted from Citrus karna. The J. Free Rad.& Antioxid. Photon, 140,265-270 (2014). (ISJN: 9102-2347, Impact Index: 5.13
Bais, S.S., Lawrence, K., Pandit S. and Lawrence, R.S.: Bio-remediation of water bodies in Allahabad by Eicchornia crassipes: Study of physico-chemical and biochemical parameters of water hyacinth. Intl. J. Res. Environ. Sci.& Tech. 4, 20-24 (2014). (ISSN 2249–9695)
Singh A., Lawrence, K., Pandit S. and Lawrence, R. S.: Response of leaves stems and roots of Withania somnifera to copper stress Intl. J. Plant, Animal, Environ. Sci. 4, 60-67 (2014). (ISSN 2231–4490)
Bais, S.S., Lawrence, K. and Nigam, V.: Analysis of heavy metals removal by Eichhorniacrassipes (mart.) Solms World J. Pharm.& Pharma. Sci. 4, 732-739(2015). (ISSN 2278 – 4357)
Singh A., Lawrence, K., Pandit S. and Lawrence, R.S.: Oxidative stress in leaves, stems and roots of Withania somnifera on Copper exposure Int. J. Pharm. Sci. Rev. Res., 30.39-246 (2015).( ISSN 0976 – 044X)
Khatun Z., Lawrence, R.S., Jalees, M. and Lawrence, K.: Green synthesis and anti-bacterial activity of silver oxide nanoparticles prepared from Pinus longifolia leaves extract. Intl. J. of Advanced Res., 3, 337 - 343(2015). ( ISSN 2320-5407)
Mishra, A., Lawrence, R., Lawrence, K., Singh R., Amogh S. K. and Pandey, A.: Evaluation of anti-oxidant activity of polyphenol in the rhizome of Curcuma amada. Int. J. Chem. Pharm. Rev. Res. 2, 25-29 (2015). (ISSN No: 2395-3306)
Raj, A., Lawrence, R.S, Jalees, M. and Lawrence, K.: Anti-bacterial activity of zinc oxide nanoparticles prepared from Brassica oleraceae leaves extract ISSN 2320-5407 Intl. J. Adv. Res. 3, 322 – 328 (2015). (ISSN 2320-5407)
Pitambar, N., Lawrence, R.S. and Lawrence, K.: Immobilization of glucose oxidase on synthesized super paramagnetic Fe3O4 nanoparticles and evaluation of antimicrobial activity of Fe3O4 nanoparticles and Fe3O4 -GOX hybrid. Int. J. Chem. Res. 05, 23-30 (2015). (ISSN: 2249-0329)

Book/Chapter:

Lawrence, K. and Lawrence, R.: Antimicrobial activity of fruit waste. In, Antibacterial activity in natural and synthetic compounds. Eds. Pandey, A. & Gupta, R.K. (Aravind Prakashan, 2013) 49-57. (ISBN978-81-88492-57-2)
Lawrence, R.S. and Lawrence, K.: Methods for evaluating antioxidant activity.In, Role of antioxidants in health and disease prevention. Eds. Pandey, A. & Agarwal, B. (Anubhav Publishing House, 2016) -in press. (ISBN 978-93-89134-75-8)

Other:

1. GENBANK accession numbers: Pandit, S. and Lawrence, K. (2013):

Seq1 KF716164 for Aspergillus flavus

Seq2 KF716165 for Aspergillus japonicas

2.GENBANK accession numbers: Upadhyay, S., Suresh, M., Anantharaman. P. and Lawrence, K. (2015):

Seq 3 KR733098 for Odontella aurita
Seq 4 KR733100 for Chaetoceros muellerai

Conferences

Title	Sponsored by	Date	Place
Advances in Transgenic Technology and its Impact on the National Economy	SHIATS	23rd April, 2010	Allahabad
Analysis of Immobilization & Expression of Therapeutic protein extracted from algal species	SHIATS	25th – 26th November, 2013	Allahabad
Future prospects of advancements in Biological sciences, Health issues & Environmental protection	Indira Gandhi Pratisthan	07th – 08th February, 2014	Lucknow
Nutraceuticals and Indian cuisine	SHIATS	24th April, 2015	Allahabad
Bioinformatics Panorama in Agriculture and Health (NCBPAH-2015)	SHIATS	05th – 06th October, 2015	Allahabad

Teaching Experience

1. Part-time lecturer at Isabella Thoburn College, Lucknow, UP., INDIA from September 1995 till March 1996. Work load involved introducing and teaching elements of Biochemistry to 1st year under-graduate students (B.Sc.-I) under a special UGC scheme.

2. Currently Professor & Head, Dept. of Molecular & Cellular Engineering
Engineering, Jacob School of Biotechnology & Bioengineering, SHIATS, Allahabad 21107, U.P., INDIA.

3. Teaching since at AAI/AAIDU/SHIATS since March 1996 till date. Teaching work load involves conducting practicals and teaching Biochemistry, Animal Biochemistry, Clinical Biochemistry, Plant Biochemistry and Enzymology; aspects of Molecular Cell Biology to Under-graduate (B.Sc. (Ag), B.Sc (Home Economics), B. Tech.(Biotech.), B.Sc. Hons.(Biochem./Biotech/Microbiol.), Post-graduate students M.Sc.(Agril. Biochemistry, Food Science, Biochem. and Biotech.) and doctoral students. Supervision of experiments and guidance in writing of dissertation of M.Sc (Agri. Biochem./Biochem./Biotech), M..Phil. and doctoral students.

4. Served as Head, Dept. of Biochemistry & Biochemical Engineering from July 2009 till July 2012.

5. Advisor for PhD.: 5 students pursuing their doctoral thesis in Biochemistry.

International Experience: Two years as Associate Professor (October 2006 till
September 2008) at Dental College, Zleiten, Al Mergeb University, Al Khums, Libya. Work load included teaching Biochemistry to first year students of Dental College.

Faculty Profile